Procedure

Pre Experimental Preparation:
Tank Construction:
  1. Construct two tanks with the dimensions of 31x31x52 cm (Taylor 1988). These dimensions can be changed.
    1. Buy three 10 gallon fish tanks.
    2. Take off all the plastic base on all the tanks.
    3. Using wire or an exact-O knife the insulator on two of the tanks saw off the side that is square shaped.
    4. Take apart the third tank completely. There will be six parts.
    5. Using aquarium glue, take the one of the longest pieces from tank three and glue it to tank one.
    6. Repeat step E for the second tank.
    7. Give the glue enough time to fully cure (Approximate time: 7 days. This may very).
    8. Label tank one A and tank two B.
  2. Check to see if there are any leaks in the two tanks.
    1. Place both tanks on a smooth surface.
    2. Fill both tanks all the way up with water.
    3. Wait about 30 minutes and see if there is any water leaks
    4. If there is then place aquarium glue where the leak is.
    5. Repeat step D for the second tank.
    6. Give the glue enough time to fully cure (Approximate time: 7 days. This may vary).
    7. Label tank one A and tank two B.
Tank Preparation:
  1. Preparations inside the tank:
    1. Fill the tank with 38 cm of mud.
    2. Create a artificial land crab burrows that is 10cm diameter and 36cm deep (Taylor 1988).
Fish Acquisition and Tagging:
  1. Clip group As adipose fin. This will let you know which R. mar is from what group.
Experimental Procedure:
  1. Get 3 from one intraspecies R. mar. This will be group A.These were obtained from a labratory at FIT (Florida Institution of Technoligy).
  2. Get 3 from one intraspecies R. mar., but not the same. These were obtained from a labratory at FIT (Florida Institution of Technoligy). R. mar that is in group A. This will be group B.
  3. Place one R. mar from group A in tank one; place one R. mar from group B in tank two.
    1. Place fish food in the tank for food.
    2. Film, observe, and record their fin actons, Where they are in the tank (help from the grid), cashing, niping/biting, and motin of swimming.
    3. Film for 24hrs using day and night.
    4. Watch all the film (donŐt take 5 min out of every hour)
    5. Perform two additional trials.
    6. Do the tanks simultaneously.
  4. Place one R . mar from group A in tank one and one R. mar from Group B in tank two.
    1. Place fish food in the tank for food.
    2. Film, observe, and record their fin actons, Where they are in the tank (help from the grid), cashing, niping/biting, and motin of swimming.
    3. Film all night and take five minutes from every hour out of the footage and observe it carefully.
    4. Continue experiment over a period of three days.
    5. Perform two additional trials.
    6. Do the tanks simultaneously.
  5. Place two R . mar from group A in tank one and two R. mar from Group B in tank two.
    1. Place fish food in the tank for food.
    2. Film, observe, and record their fin actons, Where they are in the tank (help from the grid), cashing, niping/biting, and motin of swimming.
    3. Film all night and take five minutes from every hour out of the footage and observe it carefully.
    4. Continue experiment over a period of three days.
    5. Perform two additional trials.
    6. Do the tanks simultaneously.
Care and feeding of fish:
  1. Proper care of the fish is used throughout the entire experiment:
    1. Feed the fish once every two days.
    2. Keep the water at 25ppt (parts per thousand).
    3. Keep one fish in each culture dish.
    4. When the culture dish's water becomes dirty put the same salinity and amount of water in each culture dish.
    5. Stack the culture dishes on top of each other (three finger bowls per stack) and place an extra finger bowl on the top of the stack (this will prevent the fish from jumping out of the finger bowls.
    6. At the end of experiment fish will be returned to Dr. Taylor.