Procedure


Culturing Condylactis

Step 1: Obtain four ten gallon tanks to culture the Condylactis (So that the Condylactis are not crowded in one tank)

Step 2: Equip each tank with an undergravel filter system.

Step 3: Spread crushed oyster shells along the bottom of the tank.(oyster shells help the pH remain a constant)

Step 4: Set atinic day bulbs on a timer for 16 hrs. of light per day.

Step 5: Put 5 ml of detoxifier (AmQuel) in the water for every ten gallons of water to remove cholorine.

Step 6: Adjust specific gravity (salinity) of the water to 1.024.

Step 7: Add water.

Step 8: Float the Condylactis, while still in their bags, in the water for ten to fifteen minutes so they can adjust to the temperature.

Step 9: Take Condylactis out of their bags and put in the water.


The Temperature Variation and Duration Experiment

Step 1: Put one heater in each of the four tanks.

Step 2: Adjust the tanks to these temperatures : The first tank will be 18 degrees C, the next 25 degrees C, and the last 31 degrees C.

Step 3: Count the number of Condylactis you have then divide that by 4 for and put them in the tanks so you have an equal amount of Condylactis in each group.

Step 4: Take zooxanthellae counts at the following times: 0 hours, 1 hour, 6 hours, 12 hours, and 24 hours of exposure to the temperature changes.


Zooxanthellae Counts

Step 1: Take one Condylactis out of the tank you are getting the zooxanthellae counts for and put it in a culture dish.

Step 2: Put a paper towel on a watch glass to put the tentacles on. (The paper towel is to soak up the water from inside the tentacle so the water is not counted in the mass of the tentacles)

Step 3 :Cut about one half a gram of the tentacles off and place on a watch glass. (Only take about 5 tentacles from each Condylactis)

Step 4: Put 100 ml of clean sea water in a blender.

Step 5: Homogenize (Blend) the tentacles in the 100 ml of clean sea water in the blender.

Step 6: Immediately take a one drop sample of the homogenized tentacles and put it on a slide.

Step 7: Put the slides under a microscope.

Step 8: Move the slide around to 3 different places and count the zooxanthellae in each field.

Step 9: After counting the zooxanthellae for three slides and three fields per slide determine the mean for each group.

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