The purpose of this study is to investigate the effects of temperature on the action of a restriction enzyme.
DNA fragments cut with a restriction enzyme maintained at 60 degrees C will produce the smallest fragments as indicated by electrophoresis.
Enzymes are special proteins that affect
the rate of chemical reactions. Enzymes are made by saliva, the stomach, the
pancreas, and the small intestines. In the process of digestion the enzymes
become very active. Enzymes help split larger molecules into smaller ones.
In 1822, William Beaumont, a U.S. army surgeon, had a very unique chance to study the digestive system because a soldier was shot in the stomach. With the cooperation of the patient, Dr. Beaumont watched the enzymes do their work. He found out that enzymes were a protein and the in lining of the stomach is made up of chemical resistant tissue.
. Without enzymes food would digest very slowly. Temperature can change the structure of the enzymes so that they do not function.
Enzymes can increase the rate of digestion up to a trillion times faster than the same reaction without enzymes. Enzymes reaction time increases when thermal energy is applied until it reaches a certain point and then changes the structure of the enzyme. There are many factors that can affect the enzyme reaction rate. Variations in the concentration of substrate molecules, temperature, and pH, just name a few, are all factors that affect enzymes.
I. Making the agrose gel:
The data did support the hypothesis. Group C (60 degrees C.) did have the longest lines in the gel.